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Genetic methods in honey bee breeding ; Генетические методы в селекции медоносной пчелы

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  • Additional Information
    • Contributors:
      The study was supported by the grant of the Russian Science Foundation No. 22-74-00004 (https://rscf.ru/project/22-74-00004/) using the resources of the Center for Collective Use of the UFRC RAS.
    • Publication Information:
      Institute of Cytology and Genetics of Siberian Branch of the RAS
    • Publication Date:
      2023
    • Collection:
      Vavilov Journal of Genetics and Breeding / Вавиловский журнал генетики и селекции
    • Abstract:
      The honey bee Apis mellifera is a rather difficult object for selection due to the peculiarities of its biology. Breeding activities in beekeeping are aimed at obtaining bee colonies with high rates of economically useful traits, such as productivity, resistance to low temperatures and diseases, hygienic behavior, oviposition of the queen, etc. With two apiaries specializing in the breeding of A. m. mellifera and A. m. carnica as examples, the application of genetic methods in the selection of honey bees is considered. The first stage of the work was subspecies identification based on the analysis of the polymorphism of the intergenic mtDNA locus tRNAleu-COII (or COI-COII) and microsatellite nuclear DNA loci Ap243, 4a110, A24, A8, A43, A113, A88, Ap049, A28. This analysis confirmed that the studied colonies correspond to the declared subspecies. In the apiary with A. m. mellifera, hybrid colonies have been identified. A method based on the analysis of polymorphisms of the tRNAleu-COII locus and microsatellite nuclear DNA loci has been developed to identify the dark forest bee A. m. mellifera and does not allow one to differentiate subspecies from C (A. m. carnica and A. m. ligustica) and O (A. m. caucasica) evolutionary lineages from each other. The second stage was the assessment of the allelic diversity of the csd gene. In the apiary containing colonies of A. m. mellifera (N = 15), 20 csd alleles were identified. In the apiary containing colonies of A. m. carnica (N = 44), 41 alleles were identified. Six alleles are shared by both apiaries. DNA diagnostics of bee diseases showed that the studied colonies are healthy. Based on the data obtained, a scheme was developed for obtaining primary material for honey bee breeding, which can subsequently be subjected to selection according to economically useful traits. In addition, the annual assessment of the allelic diversity of the csd gene will shed light on the frequency of formation of new allelic variants and other issues related to the evolution of this gene. ...
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    • Relation:
      https://vavilov.elpub.ru/jour/article/view/3779/1716; Bakonyi T., Derakhshifar I., Grabensteiner E., Nowotny N. Development and evaluation of PCR assays for the detection of Paenibacillus larvae in honey samples: comparison with isolation and biochemical characterization. Appl. Environ. Microbiol. 2003;69(3):1504­1510. DOI 10.1128/AEM.69.3.1504­1510.2003.; Berényi O., Bakonyi T., Derakhshifar I., Köglberger H., Nowotny N. Occurrence of six honeybee viruses in diseased Austrian apiaries. Appl. Environ. Microbiol. 2006;72(4):2414­2420. DOI 10.1128/AEM.72.4.2414­2420.2006.; Bertrand B., Alburaki M., Legout H., Moulin S., Mougel F., Garnery L. MtDNA COI­COII marker and drone congregation area: an efficient method to establish and monitor honeybee (Apis mellifera L.) conservation centers. Mol. Ecol. Resour. 2015;15(3):673­683. DOI 10.1111/1755­0998.12339.; Beye M., Hasselmann M., Fondrk M., Page R.E., Omholt S.W. The gene csd is the primary signal for sexual development in the honeybee and encodes an SR­type protein. Cell. 2003;114(4):419­429. DOI 10.1016/S0092­8674(03)00606­8.; Cridland J.M., Tsutsui N.D., Ramírez S.R. The complex demographic history and evolutionary origin of the western honey bee, Apis mellifera. Genome Biol. Evol. 2017;9(2):457­472. DOI 10.1093/gbe/evx009.; De La Rúa P., Jaffé R., Dall’olio R., Muñoz I., Serrano J. Biodiversity, conservation and current threats to European honeybees. Apidologie. 2009;40:263­284. DOI 10.1051/apido/2009027.; Earl D.A., von Holdt B.M. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv. Genet. Resour. 2012;4(2):359­361. DOI 10.1007/s12686­011­9548­7.; Espregueira Themudo G., Rey­Iglesia A., Robles Tascon L., Jensen A.B., da Fonseca R.R., Campos P.F. Declining genetic diversity of European honeybees along the twentieth century. Sci. Rep. 2020;10(1):10520. DOI 10.1038/s41598­020­67370­2.; Garnery L., Franck P., Baudry E., Vautrin D., Cornuet J.­M., Solignac M. Genetic diversity of the west European honey bee (Apis melli fera mellifera and A. m. iberica). I. Mitochondrial DNA. Genet. Sel. Evol. 1998;30(Suppl.1):S31. DOI 10.1186/1297­9686­30­S1­S31.; Govan V.A., Brozel V., Allsopp M.H., Davison S. A PCR detection method for rapid identification of Melissococcus pluton in honeybee larvae. Appl. Environ. Microb. 1998;64(5):1983­1985. DOI 10.1128/AEM.64.5.1983­1985.1998.; Hyink O., Laas F., Dearden P. Genetic tests for alleles of complementary-sex-determiner to support honeybee breeding programmes. Apido logie. 2013;44(3):306­313. DOI 10.1007/s13592­012­0181­6.; Il’yasov R.A., Petukhov A.V., Poskryakov A.V., Nikolenko A.G. Local honeybee (Apis mellifera mellifera L.) populations in the Urals. Russ. J. Genet. 2007;43(6):709­711. DOI 10.1134/S1022795407060166.; James R.R., Skinner J.S. PCR diagnostic methods for Ascosphaera infections in bees. J. Invertebr. Pathol. 2005;90(2):98­103. DOI 10.1016/j.jip.2005.08.004.; Kaskinova M.D., Gaifullina L.R., Saltykova E.S., Poskryakov A.V., Nikolenko A.G. Dynamics of the genetic structure of Apis mellifera populations in the Southern Urals. Russ. J. Genet. 2022;58(1):36­41. DOI 10.1134/S1022795422010045.; Kaskinova M.D., Gataullin A.R., Saltykova E.S., Gaifullina L.R., Poskryakov A.V., Nikolenko A.G. Polymorphism of the hypervariable region of the csd gene in the Apis mellifera L. population in Southern Urals. Russ. J. Genet. 2019;55(2):267­270. DOI 10.1134/S102279541902008X.; Martín­Hernández R., Meana A., Prieto L., Salvador A.M., Garrido­Bailón E., Higes M. Outcome of colonization of Apis mellifera by Nosema ceranae. Appl. Environ. Microbiol. 2007;73(20):6331­6338. DOI 10.1128/AEM.00270­07.; Meixner M.D., Pinto M.A., Bouga M., Kryger P., Ivanova E., Fuchs S. Standard methods for characterising subspecies and ecotypes of Apis mellifera. J. Apic. Res. 2013;52(4):1­28. DOI 10.3896/IBRA.1.52.4.05.; Mroczek R., Laszkiewicz A., Blazej P., Adamczyk­Weglarzy K., Niedbalska­Tarnowska J., Cebrat M. New insights into the criteria of functional heterozygosity of the Apis mellifera complementary sex determining gene – discovery of a functional allele pair differing by a single amino acid. PLoS One. 2022;17(8):e0271922. DOI 10.1371/journal.pone.0271922.; Neumann P., Carreck N.L. Honey bee colony losses. J. Apic. Res. 2010;49(1):1­6. DOI 10.3896/IBRA.1.49.1.01.; Nikolenko A.G., Fakhretdinova S.A., Ilyasov R.A., Poskryakov A.V. The geographic range of the Burzyan population of the European dark honeybee Apis mellifera mellifera L. (Hymenoptera: Apidae). Trudy Russkogo Entomologicheskogo Obshchestva = Proceedings of the Russian Entomological Society. 2010;81(2):202­208. (in Russian); Nikolenko A.G., Poskryakov A.V. Polymorphism of locus COI-COII of mitochondrial DNA in the honeybee Apis mellifera L. from the Southern Ural region. Russ. J. Genet. 2002;38(4):364­368. DOI 10.1023/A:1015289900666.; Nikonorov I.M., Ben’kovskaya G.V., Poskryakov A.V., Nikolenko A.G., Vakhitov V.A. The use of the PCR technique for control of pure­breeding of honeybee (Apis mellifera mellifera L.) colonies from the Southern Urals. Russ. J. Genet. 1998;34(11):1344­1347.; Ruttner F. Breeding Techniques and Selection for Breeding of the Honeybee: an Introduction to the Rearing of Queens, the Conduct of Selection Procedures and the Operation of Mating Stations. Condor, Derby: British Isles Bee Breeders Assoc., 1988.; Solignac M., Vautrin D., Loiseau A., Mougel F., Baudry E., Estoup A., Garnery L., Haberl M., Cornuet J.­M. Five hundred and fifty microsatellite markers for the study of the honeybee (Apis mellifera L.) genome. Mol. Ecol. Notes. 2003;3(2):307­311. DOI 10.1046/j.1471­8286.2003.00436.x.; Tokarev Y.S., Huang W.F., Solter L.F., Malysh J.M., Becnel J.J., Vossbrinck C.R. A formal redefinition of the genera Nosema and Vairimorpha (Microsporidia: Nosematidae) and reassignment of species based on molecular phylogenetics. J. Invertebr. Pathol. 2020;169:107279. DOI 10.1016/j.jip.2019.107279.; Zareba J., Blazej P., Laszkiewicz A., Sniezewski L., Majkowski M., Janik S., Cebrat M. Uneven distribution of complementary sex determiner (csd ) alleles in Apis mellifera population. Sci. Rep. 2017;7:2317. DOI 10.1038/s41598­017­02629­9.; https://vavilov.elpub.ru/jour/article/view/3779
    • Accession Number:
      10.18699/VJGB-23-44
    • Online Access:
      https://vavilov.elpub.ru/jour/article/view/3779
      https://doi.org/10.18699/VJGB-23-44
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      Authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access). ; Авторы, публикующие в данном журнале, соглашаются со следующим:Авторы сохраняют за собой авторские права на работу и предоставляют журналу право первой публикации работы на условиях лицензии Creative Commons Attribution License, которая позволяет другим распространять данную работу с обязательным сохранением ссылок на авторов оригинальной работы и оригинальную публикацию в этом журнале.Авторы сохраняют право заключать отдельные контрактные договорённости, касающиеся не-эксклюзивного распространения версии работы в опубликованном здесь виде (например, размещение ее в институтском хранилище, публикацию в книге), со ссылкой на ее оригинальную публикацию в этом журнале.Авторы имеют право размещать их работу в сети Интернет (например в институтском хранилище или персональном сайте) до и во время процесса рассмотрения ее данным журналом, так как это может привести к продуктивному обсуждению и большему количеству ссылок на данную работу (См. The Effect of Open Access).
    • Accession Number:
      edsbas.28A88A83