Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae) ; Evaluación de Dos Crioprotectores y Tres Curvas de Congelación Programable en la Criopreservación de Semen de Brycon henni (Pisces: Characidae)

The aim of this study was to evaluate the cryopreservation of Brycon henni semen using three programmable freezing curves and two permeable cryoprotectants. Semen of 80 males were diluted in medium supplemented with ethylene glycol (EG) or dimethylsulfoxide (DMSO), and cryopreserved in semen straws using three programmable freezing curves: slow (66 min, -0.42 °C/min), medium (43.3 min, -0.6 °C/min) and ultra-rapid (7.7 min, -5.19 °C/min). After one month of storage, semen was thawed and motility was evaluated through a class analysis system (SCA®) and sperm vitality (EV) by fluorescence microscopy with SYBR14/IP probes. For statistical analysis, generalized linear models (GLM) were adjusted and means compared by the Tukey test. The medium and ultrarapid velocity curves showed higher and equivalent values for total motility, progressive motility, and linear velocity of sperm (p<0.05). Sperm vitality was better in the medium velocity curve (52.4 ± 8.6%) in comparison with the ultra-rapid (43.0 ± 19.4%) and slow (29.0 ± 11.8%) curves (p<0.05). EG showed greater sperm vitality (p<0.05). It is concluded that freezing of sabaleta semen (B. henni) by a medium velocity programmable freezing curve and ethylene glycol as cryoprotectant allows better results of post-thaw semen quality. ; El objetivo del presente estudio fue evaluar la criopreservación de semen de Brycon henni, mediante tres curvas de congelación programable y con el empleo de dos crioprotectores permeables. El semen de 30 machos se diluyó en un medio suplementado con etilenglicol (EG) o dimetilsulfóxido (DMSO) y se crioconservó en pajillas de semen, utilizando tres curvas de congelación programable: lenta (66 min, -0.42 ºC/min), media (43.3 min, -0.6 ºC/min) y ultra-rápida (7.7 min, -5.19 ºC/min). Después de un mes de almacenamiento, el semen fue descongelado y se evaluó la movilidad, mediante el sistema de análisis de clase (SCA®) y la vitalidad espermática (VE) mediante microscopía de fluorescencia con las sondas SYBR14/IP. Para el análisis ...