Contributors: Génétique du Développement de la Drosophile; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS); Biophysique et Neuroscience Théoriques; Laboratoire de physique de l'ENS - ENS Paris (LPENS); Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Département de Physique de l'ENS-PSL; École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Département de Physique de l'ENS-PSL; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL); This work was funded by the Agence Nationale de la Recherche (ANR-10-LABX-0073) and Fondation pour la Recherche Médicale (FRM-DEQ20180339219).; We thank Y. Bellaïche, A. Brand, Y. Cai, L. Cheng, D. Glover, Y. Hong, Y. N. Jan, M. Krahn, F. Matsuzaki, A. Salzberg, M. Sato, J. Skeath, M. Suzanne, A. Wodarz, the Developmental Studies Hybridoma Bank, Flybase, and Image Analysis Hub of the Institut Pasteur for reagents and resources. We thank S. Herbert (Image Analysis Hub), R. Levayer, J.-Y. Tinevez (Image Analysis Hub), T. Schweisguth, and L. Valon for help in segmentation, local z-projection, and image data analysis; M. Rujano for help in live imaging; and L. Couturier for technical help. We thank S. Chanet, E. Contreras, A. Hakes, and G. Perez-Mockus for critical reading.; ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010)
Abstract: International audience ; Many tissues are produced by specialized progenitor cells emanating from epithelia via epithelial-to-mesenchymal transition (EMT). Most studies have so far focused on EMT involving single or isolated groups of cells. Here we describe an EMT-like process that requires tissue-level coordination. This EMT-like process occurs along a continuous front in the Drosophila optic lobe neuroepithelium to produce neural stem cells (NSCs). We find that emerging NSCs remain epithelial and apically constrict before dividing asymmetrically to produce neurons. Apical constriction is associated with contractile myosin pulses and involves RhoGEF3 and down-regulation of the Crumbs complex by the E3 ubiquitin ligase Neuralized. Anisotropy in Crumbs complex levels also results in accumulation of junctional myosin. Disrupting the regulation of Crumbs by Neuralized lowered junctional myosin and led to imprecision in the integration of emerging NSCs into the front. Thus, Neuralized promotes smooth progression of the differentiation front by coupling epithelium remodeling at the tissue level with NSC fate acquisition.
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