Abstract: Zingiber odoriferum (Zingiberaceae), a critically endangered ginger species endemic to Java, Indonesia, is ecologically vital yet genomically understudied. We present the first complete chloroplast (cp) genome (163 538 bp), revealing a quadripartite structure with a large single‐copy region (88 033 bp), a small single‐copy region (15 885 bp), and a pair of inverted repeat regions (29 810 bp each), with a total GC content of 36.04%. The genome encodes 133 genes, including protein‐coding genes, tRNA genes, rRNA genes, and pseudogenized ycf 1 – a conserved Zingiberaceae feature. Leucine (10.33%) and cysteine (1.14%) are the most and least abundant amino acids, respectively. Codon usage favors A/T‐rich codons (e.g. AGA [Arg], TTA [Leu]), while C/G‐ending codons (e.g. AGC [Ser]) are underutilized. Hypervariable loci in non‐coding regions and repeats are identified as molecular markers. Phylogenetic analysis places Z. odoriferum sister to Z. teres and Z. smilesianum (100% bootstrap support). Despite high genome‐wide conservation, intergenic spacers exhibit 5–12% divergence, aiding species delineation. However, the limited cp diversity across Zingiber underscores the need for nuclear genomic integration to resolve cryptic diversity and enhance conservation strategies. This study advances genomic resources for Z. odoriferum , highlights cp genome evolutionary constraints, and provides actionable markers for biodiversity monitoring. It emphasizes the urgency of multi‐omics approaches to safeguard this species and its threatened tropical habitat.
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