Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

mScarlet3: a brilliant and fast-maturing red fluorescent protein

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Contributors:
      Swammerdam Institute for Life Sciences (SILS); University of Amsterdam Amsterdam = Universiteit van Amsterdam (UvA); Biomedical Sciences of Cells & Systems; University of Groningen Groningen; Institut de biologie structurale (IBS - UMR 5075); Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG); Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA); European Synchrotron Radiation Facility (ESRF); ANR-17-CE11-0013,BANDIT,d'une Nox bactérienne à la conception d'outil de criblage(2017)
    • Publication Information:
      CCSD
      Nature Publishing Group
    • Publication Date:
      2023
    • Collection:
      HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives)
    • Abstract:
      International audience ; We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a barrel rigidified at one of its heads by a large hydrophobic patch of internal residues. mScarlet3 behaves well as a fusion tag, displays no apparent cytotoxicity and it surpasses existing red fluorescent proteins as a Förster resonance energy transfer acceptor and as a reporter in transient expression systems.
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/36973546; PUBMED: 36973546
    • Accession Number:
      10.1038/s41592-023-01809-y
    • Online Access:
      https://hal.science/hal-04162894
      https://hal.science/hal-04162894v1/document
      https://hal.science/hal-04162894v1/file/Gadella%20et%20al.%20NatMethods.pdf
      https://doi.org/10.1038/s41592-023-01809-y
    • Rights:
      info:eu-repo/semantics/OpenAccess
    • Accession Number:
      edsbas.76506CFE