Identification of key transcription factors at early (C1) and late (E1) molting stages.

Aggregate ATAC-seq footprint plot for GRH (a) , ECR-USP (b) , C/EBP (c) and βFtz-f1 (d) obtained over binding sites within the genome. For (a) and (b) , the open chromatin regions at C1 stage were used to do the motif enrichment. Then the footprinting analysis were performed and visualized to detect the characteristic “footprint” pattern (depletion of cleavage events in the bound region) around the motif instances. (e) Correlation coefficient between variation in gene expression of the indicating transcription factors and their potential targeted genes. (f) GO terms enrichment for the targeted genes of the transcription factors. (g) Genome browser shot of normalized ATAC and RNA sequencing counts around βFtz-f1 gene. The C/EBP binding site is highlighted in pink. (h) Genome browser shot of normalized ATAC and RNA sequencing counts around Cyp18a1 gene. The βFtz-f1 binding site is highlighted in pink.