Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Making Invisible RNA Visible: Discriminative Sequencing Methods for RNA Molecules with Specific Terminal Formations

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Publication Information:
      MDPI AG
    • Publication Date:
      2022
    • Collection:
      Directory of Open Access Journals: DOAJ Articles
    • Abstract:
      Next generation sequencing of RNA molecules (RNA-seq) has become a common tool to characterize the expression profiles of RNAs and their regulations in normal physiological processes and diseases. Although increasingly accumulating RNA-seq data are widely available through publicly accessible sites, most of the data for short non-coding RNAs (sncRNAs) have been obtained for microRNA (miRNA) analyses by standard RNA-seq, which only capture the sncRNAs with 5′-phosphate (5′-P) and 3′-hydroxyl (3′-OH) ends. The sncRNAs with other terminal formations such as those with a 5′-hydroxyl end (5′-OH), a 3′-phosphate (3′-P) end, or a 2′,3′-cyclic phosphate end (2′,3′-cP) cannot be efficiently amplified and sequenced by standard RNA-seq. Due to the invisibility in standard RNA-seq data, these non-miRNA-sncRNAs have been a hidden component in the transcriptome. However, as the functional significances of these sncRNAs have become increasingly apparent, specific RNA-seq methods compatible with various terminal formations of sncRNAs have been developed and started shedding light on the previously unrecognized sncRNAs that lack 5′-P/3′-OH ends. In this review, we summarize the expanding world of sncRNAs with various terminal formations and the strategic approaches of specific RNA-seq methods to distinctively characterize their expression profiles.
    • ISSN:
      2218-273X
    • Relation:
      https://www.mdpi.com/2218-273X/12/5/611; https://doaj.org/toc/2218-273X; https://doaj.org/article/f3941d2cac584e45b945e5c3809fee0c
    • Accession Number:
      10.3390/biom12050611
    • Online Access:
      https://doi.org/10.3390/biom12050611
      https://doaj.org/article/f3941d2cac584e45b945e5c3809fee0c
    • Accession Number:
      edsbas.A9651624