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Imaging Techniques in Insects

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  • Additional Information
    • Contributors:
      Institut de Biologie Paris Seine (IBPS); Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Centre des Sciences du Goût et de l'Alimentation Dijon (CSGA); Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro); Universität Wien = University of Vienna; Center for Mind/Brain Sciences (CIMEC); Università degli Studi di Trento = University of Trento (UNITN); Lesley J. Rogers; Giorgio Vallortigara
    • Publication Information:
      CCSD
      Springer US
    • Publication Date:
      2025
    • Collection:
      Université de Bourgogne (UB): HAL
    • Abstract:
      Protocol in book chapter. ; International audience ; The present chapter describes how to investigate brain lateralization in insects with methods of optical neuroimaging. It provides two complete protocols, one for in vivo imaging to obtain information on functional lateralization, and one for histochemical techniques to study morphological asymmetries. Both sections start with the animal preparation, illustrate the different possibilities for brain tissue labeling, and review the available imaging techniques, concentrating on widefield fluorescence microscopy, confocal, and two-photon laser scanning microscopy. After some remarks on the main methods for data analysis, studies on functional and morphological lateralization in insects are reviewed.
    • Accession Number:
      10.1007/978-1-0716-4240-5
    • Online Access:
      https://hal.inrae.fr/hal-04904354
      https://hal.inrae.fr/hal-04904354v1/document
      https://hal.inrae.fr/hal-04904354v1/file/paoli_2025_vol217_pp591_protocol.pdf
      https://doi.org/10.1007/978-1-0716-4240-5
    • Rights:
      http://hal.archives-ouvertes.fr/licences/copyright/ ; info:eu-repo/semantics/OpenAccess
    • Accession Number:
      edsbas.B41626C2