Development of a detection system for food hazards by aggregation between gold nanoparticles and aptamer-decorated bifunctional linkers ; 압타머 성형 이중기능링커와 금나노입자의 응집 기반 식품 내 위해요소 검출 시스템 개발

학위논문(박사) -- 서울대학교대학원 : 농업생명과학대학 농생명공학부, 2021.8. 이준엽. ; The importance of developing analytical methods for rapid detection of harmful microorganisms and harmful substances in the food industry is becoming increasingly prominent as the industry develops. Currently, analysis of hazardous substances is performed through various methods. However, there remains a need for the development of simple, convenient, and rapid detection methods, because the food industry belongs to a relatively low value-added industry and many low-skilled workers are employed. Gold nanoparticle aggregation based colorimetric detection has been attracting attention due to their simplicity, rapidity, and accuracy. However, it also has a drawback in that it exhibits lower sensitivity compared to electrochemical or optical methods. To overcome these shortcomings, a colorimetric detection method based on a bifunctional linker that exhibits improved visual signals with higher sensitivity has been proposed. However, there were still disadvantages in that the research so far has been focused on the detection of microorganisms and the diversity of the bifunctional linker is insufficient. In this study, first, a bifunctional linker-based detection method was applied to detect a new target, Ara h 1, a peanut allergen. The target allergen was rapidly detected below the ED01 threshold, which is the minimum that can be recognized by allergy patients in cookies. By showing that it could detect less than 0.19 mg/mL within 45 minutes, including the extraction time, it was shown that the detection system based on the bifunctional linker can be applied to the detection of various hazards beyond microorganisms. Second, a novel bifunctional linker based on an aptamer, a DNA sequence that could selectively recognize a target, and a colorimetric detection method using this linker were devised. The bifunctional linker was designed by decorating the surface of silica nanoparticles with an aptamer, and on the contrary, for the gold nanoparticles, a DNA sequence ...