Contributors: Institut de Recherche en Horticulture et Semences (IRHS); Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro); Laboratoire des Interactions Plantes Microbes Environnement (LIPME); Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Immunology and New Concepts in ImmunoTherapy (INCIT); Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE); Nantes Université - pôle Santé; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ); This research was funded by a grant (to A.B.) from INRAE-BAP department and the region Pays de la Loire, and supported by the regional program "Objectif Vegetal, Research, Education and Innovation in Pays de la Loire", supported by the region Pays de la Loire, Angers Loire Metropole and the European Union. The authors are grateful for the technical support provided by platforms COMIC (bacterial collection), IMAC (cell imaging) and Phenotic (greenhouse facilities) of the SFR QUASAV.
Abstract: International audience ; Lectins, a large group of proteins present in all kingdoms of life can bind reversibly to glycans. The roles of plant lectins are diverse and include resistance to biotic or abiotic stress, notably bacterial resistance. A gene family encoding amaranthin-like lectins termed MdAGGs in apple (Malus domestica) has been identified to be overexpressed upon treatment with the plant resistance inducer acibenzolar-S-methyl (ASM) which promotes enhanced resistance to the fire blight disease caused by Erwinia amylovora (Ea). In this study, we first screened the ability of purified MdAGG10 to agglutinate bacterial cells in vitro among a range of bacterial species. Several bacterial species, either Gram positive or negative, either plant- or human-pathogens were found to be agglutinated by MdAGG10 in acidic conditions. Apple and Arabidopsis lines constitutively overexpressing MdAGG10 were generated and evaluated for their resistance to, respectively, Ea and Ralstonia solanacearum, both plant pathogens that were found in our screening. Despite MdAGG10 protein accumulated in tissues of both apple and Arabidopsis lines, they remained susceptible to their respective pathogens. Interestingly, in vitro agglutination of Ea by MdAGG10 did not impair bacterial growth, suggesting that other plant molecules are involved in the resistance to fire blight triggered after an ASM treatment.
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