Određivanje sadržaja luteina u hrani za nesilice ; Determination of lutein content in laying hens feed

Lutein je antioksidans koji je bitan za ljudsko zdravlje te ga je potrebno unositi u organizam. Nalazimo ga u jajima, a količina luteina u jajima ovisi o količini luteina u hrani za nesilice. Ispitivana je najpovoljnija metoda priprema uzoraka koja uključuje ekstrakciju potpomognutu ultrazvukom korištenjem ultrazvučne sonde (UZVS) u trajanju od 3 min, bez zagrijavanja i pri t = 0. Pripremljeni uzorci analizirani su HPLC metodom. Uspoređena je koncentracija luteina u šest različitih smjesa hrane za nesilice koje su različitog sastava. Najveću koncentraciju luteina imala je hrana za nesilice obogaćena luteinom i drugim nutricinima a iznosila je 14,877 mg/kg hrane. Hrana za nesilice obogaćena luteinom i drugim nutricinima, ali pripremljena godinu dana ranije imala je znatno manju koncentraciju luteina koja je iznosila 3,752 mg/kg hrane. Vrlo visoku koncentraciju luteina imala je hrana za nesilice obogaćena samo luteinom te je iznosila 13,929 mg/kg hrane. Najnižu koncentraciju imala je hrana za nesilice obogaćena vitaminom E s iznosom od 0,321 mg/kg hrane. ; Lutein is an antioxidant that is important for human health and needs to be taken into the body. We find it in eggs but the amount of lutein in eggs depends on the amount of lutein in laying hens feed. The best method of sample preparation has been researched, and it includes ultrasonic assisted extraction using an ultrasonic probe (UZVS) for 3 min, without heating and at t = 0. The prepared samples were analyzed by HPLC method. We compared concentraction od lutein in six different mixtures of laying hens feed with different composition. The highest concentration of lutein was in laying hens feed enriched with lutein and other nutrients and amounted to 14.877 mg / kg of food. Laying hens food that were enriched with lutein and other nutrients, but prepared a year earlier had a significantly lower concentration of lutein, which was 3.752 mg / kg of food. Laying hens food that is enriched only with lutein had a very high concentration of lutein and amounted to ...