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Screening of natural Wolbachia infection in Aedes aegypti, Aedes taeniorhynchus and Culex quinquefasciatus from Guadeloupe (French West Indies)

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  • Additional Information
    • Contributors:
      Institut Pasteur de la Guadeloupe; Pasteur Network (Réseau International des Instituts Pasteur); Centre National d'Expertise sur les Vecteurs; Agence régionale de santé Guadeloupe (ARS); Agence Régionale de la Santé (ARS); Processus Infectieux en Milieu Insulaire Tropical (PIMIT); Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS); This work was supported by the Grand Port Maritime of Guadeloupe, by a FEDER grant, financed by the European Union and Guadeloupe Region (Programme Opérationnel FEDER-Guadeloupe-Conseil Régional 2014–2020, Grant number 2015-FED-192), by FEDER RESMALIN2 and by the National Center of Expertise on Vectors (Montpellier, France).; European Project: 2015-FED-192,FEDER-Guadeloupe
    • Publication Information:
      CCSD
      Elsevier
    • Publication Date:
      2018
    • Collection:
      Université de la Réunion: HAL
    • Abstract:
      International audience ; Guadeloupe islands are threatened by several mosquito-borne viruses such as Dengue, Chikungunya, Zika and West Nile virus. It appears essential to look for alternative mosquito control methods such as the incompatible insect technique (ITT) aiming at sterilizing wild females by inundative releases of incompatible males. Before considering the implementation of such a strategy, the characterization of genetic diversity of the endocellular bacterium Wolbachia regarding the local mosquito populations is a critical issue. Here, for the first time, we describe the prevalence and diversity of Wolbachia in natural populations of three mosquito species from Guadeloupe: Aedes aegypti, Aedes taeniorhynchus and Culex quinquefasciatus. The detection of Wolbachia in natural Ae. aegypti, Ae. taeniorhynchus and Cx. quinquefasciatus populations was conducted by studying Wolbachia 16S ribosomal RNA gene using a TaqMan quantitative real-time PCR and results were confirmed by conventional PCR and sequencing. In addition, molecular typing of wPip strains in Cx. quinquefasciatus was done by PCR-RFLP. We did not find Wolbachia infection in any of Ae. aegypti and Ae. taeniorhynchus studied populations. Natural Wolbachia infection was detected in Cx. quinquefasciatus with prevalence varying from 79.2% to 95.8%. In addition, no polymorphism was found between the Wolbachia strains infecting Cx. quinquefasciatus specimens, all carrying an infection from the same Wolbachia genetic wPip-I group. These results pave the way for the evaluation of the feasibility of IIT programs to fight against these medically-important mosquito species in Guadeloupe.
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/29908171; info:eu-repo/grantAgreement//2015-FED-192/EU/Programme Opérationnel FEDER-Guadeloupe-Conseil Régional 2014-2020/FEDER-Guadeloupe; PUBMED: 29908171
    • Accession Number:
      10.1016/j.actatropica.2018.06.011
    • Online Access:
      https://hal.science/hal-04366110
      https://hal.science/hal-04366110v1/document
      https://hal.science/hal-04366110v1/file/1-s2.0-S0001706X18302699-main.pdf
      https://doi.org/10.1016/j.actatropica.2018.06.011
    • Rights:
      http://creativecommons.org/licenses/by-nc-nd/ ; info:eu-repo/semantics/OpenAccess
    • Accession Number:
      edsbas.F6767999