Characterization and increment of amylase production in mutant strains of Iranian native Bacillus licheniformis

Introduction:The amylase enzyme hydrolyze starch and break glycosidic internal bonds of this polysaccharides. Amylases are important industrial enzymes for a wide range of applications including starch conversion to sugar syrup, dextrin production, and use in the pharmaceutical and medical industries. Materials and methods: In this study, thermophilic bacterium was isolated and purified from the Qynarcheh hot spring in Ardabil. Desired strain suspension in nutrient broth medium was placed in front of UV rays with a wavelength of 254 nm at a distance of 1 meter for 45 seconds in the laminar air flow. The mutant strains were compared to wild type in temperature and salinity tolerance, amylase production content and different antibiotics resistance. Results: In this study, two interesting mutant strains were isolated and named B.L.2.M.1 and B.L.2.M.2. Mutations caused many changes in bacteria such as cell growth speed and enzyme production content. Differences in cell growth, production of amylase and other characters were significant at 0.05 level (Pvalue