Abstract: Diabetes mellitus is one of the most dangerous diseases in the world because every year, people with diabetes always increase. Methods development for diagnosing diabetes mellitus was carried out to obtain better results. This study aims to analyze diabetes mellitus screening methods based on the electrical properties of cells using the dielectric method. This study used 90 mice (Mus musculus) as experimental subjects. Mice were divided into six groups: one group without streptozotocin injection (Control) and five groups injected with streptozotocin. Streptozotocin doses given were 10 mg/KgBW (P1), 15 mg/KgBW (P2), 20 mg/KgBW (P3), 25 mg/KgBW (P4), and 30 mg/KgBW (P5). Mice that had experienced hyperglycemia had their dielectric constant measured using electrical impedance spectroscopy (EIS), and changes in cell morphology were observed using a binocular microscope. The results showed that the dielectric constant value of the control group was 35000, the pre-diabetes group (P1 and P2) was 35000 to 20000, while the acute diabetes mellitus group (P3, P4, and P5) was below 20000. The results of histopathological observations identified five types of specific cell damage, namely microcytes (9%), hypochromic (11%), burr cells (20%), schistocytes (20%), and macrocytic hypochromic (40%). Electrical properties of cells in the form of dielectric constants can show area dispersion at different levels of diabetes. The results of dielectric constant measurements correlate with the histopathological picture. Percentage of accumulated damage is 32% (P1); 40% (P2); 55% (P3); 65% (P4); and 74% (P5). The dielectric method can be used as an alternative method for screening diabetes mellitus based on the electrical properties of cells.
Processing Request
No Comments.