Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

NOVEL CADHERIN RECEPTOR PEPTIDE FOR POTENTIATING Bt BIOPESTICIDES

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Publication Date:
    July 9, 2009
  • Additional Information
    • Document Number:
      20090175974
    • Appl. No:
      12/272903
    • Application Filed:
      November 18, 2008
    • Abstract:
      Disclosed is a novel cadherin peptide that enhances the toxicity of Cry proteins. A novel insecticide composition comprising an effective amount of cadherin peptide having SEQ. ID. NO:2 and an effective amount of Bacillus thuringiensis Cry protein wherein the cadherin peptide comprises a Cry3Aa toxin binding region from the full-length T. molitor cadherin and has synergistic characteristics of a binary toxin potentiating Cry3 and Cry1 toxins against coleopterans and lepidopteran species, respectively
    • Inventors:
      Oppert, Brenda S. (Manhattan, KS, US); Jurat-Fuentes, Juan Luis (Knoxville, TN, US); Fabrick, Jeffrey A. (US); Oppert, Cristopher (Knoxville, TN, US)
    • Claim:
      1. An insecticide composition comprising an effective amount of cadherin peptide having SEQ. ID. NO: 2 and an effective amount of Bacillus thuringiensis Cry protein.
    • Claim:
      2. The insecticide composition of claim 1 wherein the cadherin peptide has 90% amino acid sequence identity with SEQ. ID. NO: 2.
    • Claim:
      3. The insecticide composition of claim 1 wherein the cadherin peptide and Bacillus thuringiensis Cry protein is in a molar ratio range of approximately 1:2.5 to 1:200.
    • Claim:
      4. The insecticide composition of claim 1 wherein the Cry protein is a Cry1 or Cry3 protein.
    • Claim:
      5. The insecticide composition of claim 1 wherein the composition is effective against insects of the order Coleoptera.
    • Claim:
      6. The insecticide composition of claim 5 wherein the composition is effective against Tenebrio molitor.
    • Claim:
      7. The insecticide composition of claim 1 wherein the composition is effective against insects of the order Lepidoptera.
    • Claim:
      8. The insecticide composition of claim 7 wherein the composition is effective against Pectinophora gossypiella.
    • Claim:
      9. The insecticide composition of claim 7 wherein the composition is effective against Heliothis virescens.
    • Claim:
      10. A method for inhibiting insect pests, the method comprising selecting a Bacillus thuringiensis Cry protein, potentiating said protein with an effective amount of cadherin peptide having SEQ. ID. NO: 2, and applying an effective amount of said Cry protein and cadherin peptide to the insect pest, wherein the mortality of said insect increases.
    • Claim:
      11. The method of claim 10 wherein the wherein the cadherin peptide and Bacillus thuringiensis Cry protein is in a molar ratio range of approximately 1:2.5 to 1:200.
    • Claim:
      12. The method of claim 10 wherein the Cry protein is a Cry3 protein.
    • Claim:
      13. The method of claim 10 wherein the Cry protein is a Cry1 protein.
    • Claim:
      14. The method of claim 10 wherein the insect pest is of the order coleoptera.
    • Claim:
      15. The method of claim 10 wherein the insect pest is of the order of lepidoptera.
    • Claim:
      16. An isolated nucleic acid segment coding for a Bacillus thuringiensis toxin binding site, wherein said nucleic acid segment comprises nucleotides 4,076-4,661 from SEQ ID. NO: 3, or the complement thereof.
    • Claim:
      17. An isolated polypeptide comprising a Bacillus thuringiensis toxin binding site encoded by nucleotides 4,076-4,661 from SEQ ID. NO: 3, or a fragment thereof sufficient to encode a functional protein.
    • Claim:
      18. A method for screening insecticidal receptor comprising transfecting cells to express SEQ. ID. NO: 4 or a fragment thereof sufficient to encode a functional protein, exposing said cells to a Cry toxin; and monitoring said cells for effect attributable to toxin exposure.
    • Claim:
      19. The method of claim 18 wherein the transfected cells are monitored via cytotoxicity assay.
    • Claim:
      20. A method for screening insecticidal receptor comprising transfecting cells to express base pairs 1322-1626 and 3969-4879 of SEQ. ID. NO: 3 or a fragment thereof sufficient to encode a functional protein, exposing said cells to a Cry toxin; and monitoring said cells for effect attributable to toxin exposure.
    • Current U.S. Class:
      424/780
    • Current International Class:
      01; 01; 12; 07; 12; 12; 01
    • Accession Number:
      edspap.20090175974