- Document Number:
20210007362
- Appl. No:
16/623010
- Application Filed:
May 06, 2019
- Abstract:
The present invention provides a preparation method of Beauveria bassiana (Bals.) Vuill microsclerotium and a formulation thereof, and an application of the formulation thereof, belonging to the technical field of microsclerotium formulation preparation; the Beauveria bassiana microsclerotium formulation is prepared by the following steps of: 1) mixing conidia of Beauveria bassiana with Tween 20 aqueous solution to obtain spore suspension; 2) inoculating the prepared spore suspension into a liquid induction medium for induction culture to obtain microsclerotium; 3) mixing the prepared microsclerotium with a padding for drying to obtain dried microsclerotium; 4) mixing the prepared dried microsclerotium with an auxiliary to obtain a microsclerotium formulation. The Beauveria bassiana microsclerotium formulation produced by the method has low production cost, strong product tolerance and good insecticidal activity, moreover, after rehydrated, the formulation may rapidly germinate hyphae and produce spores to infect pests.
- Claim:
1. A preparation method of Beauveria bassiana microsclerotium, comprising the following steps of: 1) mixing conidia of Beauveria bassiana with Tween 20 aqueous solution to obtain spore suspension; 2) inoculating the spore suspension in step 1) into a liquid induction medium for induction culture to obtain the microsclerotium; wherein, the volume percent of Tween 20 in the Tween 20 aqueous solution in step 1) was 0.03%-0.1%; with water as solvent, per liter of the liquid induction medium in step 2) comprises the following ingredients: 2-6 g KH2PO4, 0.3-0.9 g MgSO4.7H2O, 0.4-1.2 g NaNO3, 0.2-0.6 g CuSO4, 1.5-4.5 g yeast powder, 1-3 g peptone; 10-30 g sucrose, 2.5 mL-7.5 mL Tween 20 and 1000 mL water, pH=6.0-7.5.
- Claim:
2. The preparation method of the Beauveria bassiana microsclerotium according to claim 1, wherein, the Beauveria bassiana strain is CQBb119 and the accession number is CGMCC NO:15987.
- Claim:
3. The preparation method of the Beauveria bassiana microsclerotium according to claim 1, wherein, the spore content of the spore suspension in step 1) is 2.8×106-3.2×106 spores/mL.
- Claim:
4. The preparation method of the Beauveria bassiana microsclerotium according to claim 1, wherein, the volume ratio of the inoculated spore suspension in step 2) to the liquid induction medium is 1:(10-20).
- Claim:
5. The preparation method of the Beauveria bassiana microsclerotium according to claim 1, wherein, the induction culture temperature in step 2) is 23-31° C.; the induction culture time is 5-6 d; the oscillation or stirring frequency of the induction culture is 50-250 rpm.
- Claim:
6. A preparation method of a Beauveria bassiana microsclerotium formulation, comprising the following steps of: A) mixing a microsclerotium prepared by the method according to claim 1 with a padding for drying to obtain dried microsclerotium; B) mixing the dried microsclerotium in step A) with an auxiliary to obtain the microsclerotium formulation.
- Claim:
7. The preparation method of the Beauveria bassiana microsclerotium formulation according to claim 6, wherein the dosage form of the Beauveria bassiana microsclerotium formulation is granule.
- Claim:
8. The preparation method of the Beauveria bassiana microsclerotium formulation according to claim 6, wherein the padding in step A) is one or more of diatomite, starch and cyclodextrin; the volume ratio of the microsclerotium to the padding is 1:(2-4).
- Claim:
9. The preparation method of the Beauveria bassiana microsclerotium formulation according to claim 6, wherein the auxiliary in step B) is sucrose ester; the mass ratio of the dried microsclerotium to the sucrose ester is 1000:1-200:1.
- Claim:
10. An application of the Beauveria bassiana microsclerotium formulation obtained by the preparation method according to claim 6 in controlling various pests.
- Claim:
11. A preparation method of a Beauveria bassiana microsclerotium formulation, comprising the following steps of: A) mixing a microsclerotium prepared by the method according to claim 2 with a padding for drying to obtain dried microsclerotium; B) mixing the dried microsclerotium in step A) with an auxiliary to obtain the microsclerotium formulation.
- Claim:
12. The preparation method of the Beauveria bassiana microsclerotium formulation according to claim 11, wherein the dosage form of the Beauveria bassiana microsclerotium formulation is granule.
- Claim:
13. The preparation method of the Beauveria bassiana microsclerotium formulation according to claim 11, wherein the padding in step A) is one or more of diatomite, starch and cyclodextrin; the volume ratio of the microsclerotium to the padding is 1:(2-4).
- Claim:
14. A preparation method of a Beauveria bassiana microsclerotium formulation, comprising the following steps of: A) mixing a microsclerotium prepared by the method according to claim 3 with a padding for drying to obtain dried microsclerotium; B) mixing the dried microsclerotium in step A) with an auxiliary to obtain the microsclerotium formulation.
- Claim:
15. The preparation method of the Beauveria bassiana microsclerotium formulation according to claim 14, wherein the dosage form of the Beauveria bassiana microsclerotium formulation is granule.
- Claim:
16. The preparation method of the Beauveria bassiana microsclerotium formulation according to claim 14, wherein the padding in step A) is one or more of diatomite, starch and cyclodextrin; the volume ratio of the microsclerotium to the padding is 1:(2-4).
- Claim:
17. A preparation method of a Beauveria bassiana microsclerotium formulation, comprising the following steps of: A) mixing a microsclerotium prepared by the method according to claim 4 with a padding for drying to obtain dried microsclerotium; B) mixing the dried microsclerotium in step A) with an auxiliary to obtain the microsclerotium formulation.
- Claim:
18. The preparation method of the Beauveria bassiana microsclerotium formulation according to claim 17, wherein the dosage form of the Beauveria bassiana microsclerotium formulation is granule.
- Claim:
19. A preparation method of a Beauveria bassiana microsclerotium formulation, comprising the following steps of: A) mixing a microsclerotium prepared by the method according to claim 5 with a padding for drying to obtain dried microsclerotium; B) mixing the dried microsclerotium in step A) with an auxiliary to obtain the microsclerotium formulation.
- Claim:
20. The preparation method of the Beauveria bassiana microsclerotium formulation according to claim 19, wherein the dosage form of the Beauveria bassiana microsclerotium formulation is granule.
- Current International Class:
01; 12; 12
- Accession Number:
edspap.20210007362
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