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Pegylated Capsule-Degrading Enzyme for the Treatment of Anthrax

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  • Publication Date:
    July 28, 2022
  • Additional Information
    • Document Number:
      20220233703
    • Appl. No:
      17/577893
    • Application Filed:
      January 18, 2022
    • Abstract:
      A pegylated, circularly permuted construct of the CapD enzyme (a gamma glutamyl transferase enzyme acting as a hydrolase specific to poly-γ-D-glutamic acid) is used to treat anthrax and other bacterial infections, including but not limited to infection with strains that are resistant to available antibiotics.
    • Claim:
      1. A purified and isolated protein comprising a pegylated, circularly permuted construct of the CapD enzyme having activity against poly-γ-D-glutamic acid and/or poly-γ-DL-glutamic acid.
    • Claim:
      2. The purified and isolated protein of claim 1, wherein the pegylation is accomplished by an S334C mutation.
    • Claim:
      3. The purified and isolated protein of claim 2, comprising SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5.
    • Claim:
      4. The purified and isolated protein of claim 1, further comprising a Fc domain fused thereto.
    • Claim:
      5. The purified and isolated protein of claim 4, comprising SEQ ID NO: 4 or SEQ ID NO: 5.
    • Claim:
      6. A medicament comprising the purified and isolated protein of claim 1.
    • Claim:
      7. A method comprising administering the purified and isolated protein of claim 1 to a subject.
    • Claim:
      8. A method of treating an infection of bacteria in a subject in need thereof, the method comprising administering the purified and isolated protein of claim 1 to the subject, wherein the bacteria has a capsule.
    • Claim:
      9. The method of claim 8, wherein the capsule comprises poly-γ-D-glutamic acid or poly-γ-DL-glutamic acid.
    • Claim:
      10. The method of claim 8, wherein the bacteria comprises Bacillus anthracis or Staphylococus epidermidis.
    • Claim:
      11. A method of treating anthrax in a subject in need thereof, the method comprising administering the purified and isolated protein of claim 1 to the subject.
    • Current International Class:
      61; 61
    • Accession Number:
      edspap.20220233703