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ENZYME AGENT FOR TRANSESTERIFICATION, INCLUDING LIPASE AS ACTIVE INGREDIENT

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  • Publication Date:
    December 26, 2024
  • Additional Information
    • Document Number:
      20240425829
    • Appl. No:
      18/687533
    • Application Filed:
      August 30, 2022
    • Abstract:
      It is an object of the present invention to provide an enzyme agent for transesterification, including, as an active ingredient, a lipase having excellent heat resistance and high specificity for the 1,3-positions. According to the present invention, provided is an enzyme agent for transesterification, including, as an active ingredient, a lipase consisting of an amino acid sequence having a sequence identity of 90% or more to the amino acid sequence as set forth in SEQ ID NO: 2 or SEQ ID NO: 4.
    • Assignees:
      AMANO ENZYME INC. (Nagoya-shi, JP)
    • Claim:
      1. An enzyme agent for transesterification, comprising, as an active ingredient, a lipase consisting of an amino acid sequence having a sequence identity of 90% or more to the amino acid sequence as set forth in SEQ ID NO: 2 or SEQ ID NO: 4.
    • Claim:
      2. The enzyme agent according to claim 1, wherein the lipase is derived from Talaromyces (also referred to as Thermomyces, at times) sp. bacteria.
    • Claim:
      3. The enzyme agent according to claim 1, wherein the lipase is derived from Talaromyces thermophilus (also referred to as Thermomyces dupontii, at times).
    • Claim:
      4. A method for producing transesterified oils and fats, comprising allowing the enzyme agent according to claim 1 to act on oils and fats.
    • Claim:
      5. The method according to claim 4, wherein the enzyme agent is allowed to act on oils and fats at a temperature of 60° C. or higher.
    • Claim:
      6. A lipase consisting of an amino acid sequence having a sequence identity of 99% or more to the amino acid sequence as set forth in SEQ ID NO: 2 or SEQ ID NO: 4, wherein the enzyme activity remains even after the lipase is treated in a 50 mM PIPES buffer (pH 7.0) at 70° C. for 30 minutes.
    • Claim:
      7. A method for producing a lipase, comprising the following steps (1) and (2): (1) culturing microorganisms having an ability to generate the lipase according to claim 6; and (2) recovering the lipase from the culture solution and/or the cell mass after completion of the culture.
    • Claim:
      8. The production method according to claim 7, wherein the microorganisms are a Talaromyces thermophilus (also referred to as Thermomyces dupontii, at times) NBRC31798T strain.
    • Current International Class:
      12; 07; 11; 12
    • Accession Number:
      edspap.20240425829