- Document Number:
20250003868
- Appl. No:
18/709012
- Application Filed:
November 10, 2022
- Abstract:
A method of performing Raman spectroscopy comprising: generating wavelength shifted excitation light for exciting a Raman response from at least one sample, wherein the wavelength shifted excitation light comprises at least two excitation wavelengths; providing the wavelength shifted excitation light to the at least one sample and collecting signal light from the at least one sample; obtaining response signals from the collected signal light; processing the obtained response signals to determine a Raman response and a fluorescence response, wherein determining the Raman response uses at least one characteristic of an expected Raman response to the wavelength shifted excitation light and wherein determining the fluorescence response uses at least one characteristic of an expected fluorescence response to the shifted excitation light.
- Assignees:
The University Court of the University of Edinburgh (Edinburgh, GB)
- Claim:
1. A method of performing Raman spectroscopy comprising: generating wavelength shifted excitation light for exciting a Raman response from at least one sample, wherein the wavelength shifted excitation light comprises at least two excitation wavelengths; providing the wavelength shifted excitation light to the at least one sample and collecting signal light from the at least one sample; obtaining response signals from the collected signal light; processing the obtained response signals to determine a Raman response and a fluorescence response, wherein determining the Raman response uses at least one characteristic of an expected Raman response to the wavelength shifted excitation light and wherein determining the fluorescence response uses at least one characteristic of an expected fluorescence response to the shifted excitation light.
- Claim:
2. The method according to claim 1, wherein the at least one characteristic of the expected Raman response comprises a sparseness of the expected Raman response and wherein the at least one characteristic of the expected fluorescence response comprises a smoothness of the expected fluorescence response.
- Claim:
3. The method according to claim 1, wherein the at least one characteristic of the expected Raman response comprises a substantial portion of the Raman response having a measure of size, amplitude and/or intensity lower than a pre-determined threshold.
- Claim:
4. The method according to claim 1, wherein the at least one characteristic of the expected Raman response comprises a response having a set of known peaks.
- Claim:
5. The method according to claim 1, wherein the at least one characteristic of the expected fluorescence response comprises a change less than a pre-determined value over a neighbourhood of a wavelength.
- Claim:
6. The method according to claim 1, wherein determining the fluorescence and Raman response comprises processing the obtained response signals subject to a first constraint or condition based on the characteristics of the expected Raman response and subject to a second constraint or condition based on the characteristics of the expected fluorescence response.
- Claim:
7. The method according to claim 6, wherein at least one of a), b), c): a) the first constraint or condition causes the determined Raman response to be modelled as a sparse signal and wherein the second constraint or condition causes the determined fluorescence response to be modelled as a smooth signal; b) the first constraint or condition is applied by minimizing a measure of the size of the Raman response; c) the second constraint or condition is applied by minimizing a measure of the variability of the fluorescence response.
- Claim:
8. (canceled)
- Claim:
9. (canceled)
- Claim:
10. The method of claim 6, wherein the effect of the first and second constraints on the determined responses are controlled by respective first and second regularization parameters and wherein the method further comprises repeating the processing of the obtained response signals for different values of the first and second regularization parameters thereby to determine a plurality of Raman and fluorescence responses.
- Claim:
11. The method of claim 10 further comprising performing a selection process on the plurality of determined responses and selecting a desired Raman response and a desired fluorescence response in accordance with a predetermined set of selection rules thereby to select on one of the plurality of determined responses.
- Claim:
12. The method of claim 11, wherein the set of selection rules are based on at least one of: a comparison between the degrees to which the determined Raman and/or fluorescence responses exhibit desired characteristics of the expected Raman and/or fluorescence response; a comparison of a measure similarity between the determined Raman and fluorescence response for each set of parameters.
- Claim:
13. The method according to claim 1, wherein obtaining response signals from the collected signal light comprises performing a plurality of measurements corresponding to a plurality of excitation wavelengths and wherein processing the obtained response signals comprises applying a model that permits changes in the intensity of the Raman response and/or the fluorescence response over the plurality of measurements.
- Claim:
14. The method according to claim 1, wherein processing the obtained response signals comprises at least one of a), b): a) modelling a bias parameter representative of additive noise from the sensor, b) applying a further constraint to ensure that the determined Raman response is non-negative.
- Claim:
15. (canceled)
- Claim:
16. The method according to claim 1, wherein processing the obtained response signals comprises determining at least one property of a Raman response spectrum and at least one property of a fluorescence response spectrum, wherein at least one of a) and b): a) the at least one property of the Raman response spectrum comprises at least one property of one or more peaks, for example, size and/or wavelength of a peak and/or number of peaks; b) the at least one property of the fluorescence response spectrum comprises at least one of: the size and shape of a smooth fluorescence background, the degree of smoothness of the fluorescence response spectrum.
- Claim:
17. The method according to claim 1, wherein at least one of a), b), c): a)_generating the shifted excitation light comprises varying a temperature of a light source; b) the method further comprises determining or estimating the wavelengths of the wavelength shifted light or the shift in wavelength of the wavelength shifted light and using the determined or estimated wavelengths or shifts in wavelength to determine the Raman and fluorescence response: c) the fluorescence and Raman responses are determined simultaneously.
- Claim:
18. (canceled)
- Claim:
19. The method according to claim 1, wherein determining the Raman response comprises performing a model fitting process comprising determining a set of model parameters to minimize a loss function, wherein a value of the loss function is dependent on an estimated Raman response and an estimated fluorescence response.
- Claim:
20. (canceled)
- Claim:
21. (canceled)
- Claim:
22. The method of claim 1, wherein the shifted excitation light is provided to a first sample portion and a second sample portion, wherein the method further comprises collecting the signal light from the first and second sample portions.
- Claim:
23. The method of claim 22, wherein at least one of a), b), c): a) the first sample portion is at a first position and the second sample portion is at a second position and the method comprises providing shifted excitation light to and collected signal light from the first and second positions, b) the determined Raman response comprises a difference between the Raman response from a first sample portion and a second sample portion and/or wherein the determined fluorescence response comprises a differences between the fluorescence response from the first sample portion and the second sample portion; c) the first sample portion comprises a healthy tissue sample and the second sample comprises an unhealthy tissue sample.
- Claim:
24. (canceled)
- Claim:
25. (canceled)
- Claim:
26. The method of claim 1, wherein the method further comprises at least one of a), b), c), d): a) further processing the response signals to determine that the at least one sample is healthy and/or unhealthy b) determining the Raman response further uses further pre-determined information for an expected response, for example, peak location information; c) determining an uncertainty associated with the determined Raman and/or fluorescence response, optionally using the determined uncertainty to identify a portion of the determined response as signal and/or background; d) performing a deconvolution process on the determined response.
- Claim:
27. (canceled)
- Claim:
28. (canceled)
- Claim:
29. (canceled)
- Claim:
30. A Raman spectroscopy apparatus comprising: a shifted wavelength excitation light generator configured to generate shifted wavelength excitation light having at least two excitation wavelengths, wherein the generated shifted wavelength excitation light is for exciting a Raman response in at least one sample; a delivery path configured to deliver the generated shifted wavelength excitation light to the at least one sample; a collection path configured to collect signal light from the at least one sample; a response measurement device for obtaining response signals from the collected signal light; a processing resource configured to process the obtained response signals to determine a Raman response and a fluorescence response, wherein determining the Raman response uses at least one characteristic of an expected Raman response to the wavelength shifted excitation light and wherein determining the fluorescence response uses at least one characteristic of an expected fluorescence response to the shifted excitation light.
- Claim:
31. A computer-implemented method comprising: processing obtained response signals to determine a Raman response and a fluorescence response, wherein the obtained response signals are representative of a response to wavelength shifted excitation light of at least one sample and wherein determining the Raman response uses at least one characteristic of an expected Raman response to the wavelength shifted excitation light and wherein determining the fluorescence response uses at least one characteristic of an expected fluorescence response to the shifted excitation light.
- Claim:
32. (canceled)
- Current International Class:
01; 01; 01; 01
- Accession Number:
edspap.20250003868
Processing Request
No Comments.