- Patent Number:
11219,220
- Appl. No:
16/623010
- Application Filed:
May 06, 2019
- Abstract:
The present invention provides a preparation method of Beauveria bassiana (Bals.) Vuill microsclerotium and a formulation thereof, and an application of the formulation thereof, belonging to the technical field of microsclerotium formulation preparation; the Beauveria bassiana microsclerotium formulation is prepared by the following steps of: 1) mixing conidia of Beauveria bassiana with Tween® 20 aqueous solution to obtain spore suspension; 2) inoculating the prepared spore suspension into a liquid induction medium for induction culture to obtain microsclerotium; 3) mixing the prepared microsclerotium with a padding for drying to obtain dried microsclerotium; 4) mixing the prepared dried microsclerotium with an auxiliary to obtain a microsclerotium formulation. The Beauveria bassiana microsclerotium formulation produced by the method has low production cost, strong product tolerance and good insecticidal activity, moreover, after rehydrated, the formulation may rapidly germinate hyphae and produce spores to infect pests.
- Inventors:
CHONGQING UNIVERSITY (Chongqing, CN)
- Assignees:
CHONGQING UNIVERSITY (Chongqing, CN)
- Claim:
1. A method for preparation of Beauveria bassiana microsclerotium, comprising the following steps of: 1) mixing conidia of Beauveria bassiana with an aqueous solution comprising polyoxyethylene (20) sorbitan monolaurate to obtain a spore suspension; and 2) inoculating the spore suspension obtained in step 1) into a liquid induction medium for induction culture to obtain the microsclerotium; wherein, the volume percent of polyoxyethylene (20) sorbitan monolaurate in the aqueous solution in step 1) is 0.03%-0.1%; with water as solvent, per liter of the liquid induction medium in step 2) having a pH of 6.0-7.5 comprises the following ingredients: 2-6 g KH 2 PO 4 , 0.3-0.9 g MgS0 4 .7H 2 0, 0.4-1.2 g NaNO3, 0.2-0.6 g CuSO 4 , 1.5-4.5 g yeast powder, 1-3 g peptone; 10-30 g sucrose, and 2.5 mL polyoxyethylene (20) sorbitan monolaurate; the Beauveria bassiana strain is CQBb119 and the accession number is CGMCC NO: 15987; the spore content of the spore suspension in step 1) is 2.8×10 6 -3.2×10 6 spores/mL; the volume ratio of the inoculated spore suspension in step 2) to the liquid induction medium is 1:10 to 1:20; and the induction culture temperature in step 2) is 23-31° C.; the induction culture time period is 5-6 days; and an oscillation or stirring frequency of the induction culture is 50-250 rpm.
- Claim:
2. A method for preparing a formulation comprising Beauveria bassiana microsclerotium, comprising the following steps of: A) mixing the microsclerotium prepared by the method as set forth in claim 1 with a padding for drying to obtain dried microsclerotium; and B) mixing the dried microsclerotium obtained in step A) with an auxiliary to obtain the microsclerotium formulation.
- Claim:
3. The method according to claim 2 , wherein a dosage form of the microsclerotium formulation is granule.
- Claim:
4. The method according to claim 2 , wherein the padding in step A) is one or more selected from the group consisting of diatomite, starch and cyclodextrin; and the volume ratio of the microsclerotium to the padding is 1:2 to 1:4.
- Claim:
5. The method according to claim 2 , wherein the auxiliary in step B) is a sucrose ester; and the mass ratio of the dried microsclerotium to the sucrose ester is 1000:1-200:1.
- Patent References Cited:
2002/0146444 October 2002 Bradley et al.
2016/0183532 June 2016 Taghavi et al.
101245319 August 2008
102206587 October 2011
104357338 February 2015
105695344 June 2016
108949588 December 2018
- Other References:
Wang Haihong et. al., 2011—Corresponding to CN102206587A cited in applicant's IDS dated Apr. 19, 2021—“Culture method of sclerotium of Beauveria bassiana and application thereof”, English machine-translation (total pp. 1-16). (Year: 2011). cited by examiner
Yuan et al., “Screening of Higher Virulent Fungal Strain against Delia antigua”, Chinese Journal of Biological Control, Jan. 2016, vol. 32, No. 2, pp. 165-171 (total pp. 1-7 with English ABSTRACT). (Year: 2016). cited by examiner
Wang Jinyao et al., 2008—Corresponding to CN101245319A cited in applicant's IDS dated Apr. 19, 2021, “Macrophomina Beauveria bassiana HFW-05 bacterial strain and uses thereof”, English machine-translation (total pp. 1-14). (Year: 2008). cited by examiner
First Office Action dated Apr. 3, 2020 issued in Chinese Patent Application No. 201810968356.2. cited by applicant
- Primary Examiner:
Singh, Satyendra K
- Attorney, Agent or Firm:
Troutman Pepper Hamilton Sanders LLP
Close, Jr., Christopher C.
- Accession Number:
edspgr.11219220
Processing Request
No Comments.