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Cucumber male sterility gene, molecular marker, screening method and application thereof

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  • Publication Date:
    May 03, 2022
  • Additional Information
    • Patent Number:
      11319,600
    • Appl. No:
      16/610853
    • Application Filed:
      January 12, 2018
    • Abstract:
      The invention relate to SNP and InDel markers associated with a cucumber male sterility gene and a screening method thereof. The cucumber male sterility gene is acquired by screening according to the markers. Specifically, the screening method includes following steps: (1) configuring groups; (2) building a library and sequencing; (3) adopting bulked segregation analysis (BSA); and (4) performing competitive allele specificity PCR (KASP), finely mapping a sterility gene, and acquiring SNP or InDel locus in close linkage with sterility character. The acquiring of the male sterility gene provides an effective tool for quick identification of cucumber male sterility plants and efficient production of cucumber hybrid species.
    • Inventors:
      Tianjin Kernel Agricultural Science and Technology Corporation Ltd. Cucumber Research Institute (Tianjin, CN)
    • Assignees:
      Tianjin Kernel Agricultural Science and Technology Corporation Ltd. Cucumber Research Institute (Tianjin, CN)
    • Claim:
      1. A cucumber male sterile gene comprising a nucleic acid sequence comprising SEQ ID NO: 3.
    • Patent References Cited:
      102260338 November 2011
      103525917 January 2014
      104120126 October 2014
      105420408 March 2016
      105525000 April 2016
      107058552 August 2017
      WO 2016/086305 June 2016







    • Other References:
      GenBank (2015) Reference Sequence XM_011654407.2, 3pp. (Year: 2015). cited by examiner
      Theoretical and Applied Genetics (2018) 131:449-460, including supplemental Fig S2. published online Nov. 13, 21017. (Year: 2017). cited by examiner
      International Search Report and Written Opinion dated Apr. 9, 2018, for International application No. PCT/CN2018/072412, from which the present application claims priority. cited by applicant
      Liang (Published online Oct. 2016) “Map-based Cloning of the Dominant Genic Male Sterile Ms-cdl Gene in Cabbage (Brassica oleracea),” Theoretical and Applied Genetics (Jan. 2017) 130, pp. 71-79. cited by applicant
      Chinese First Office Action and Search Report with English translation, dated Apr. 1, 2020, in Chinese Patent Application No. 201710313270.1, 11 pp. cited by applicant
      Han et al. (Nov. 2017) “Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant,” Theoretical and Applied Genetics (2018) 131:449-460, 12 pp. cited by applicant
      Lu (2014) “QTL Mapping of Early Flowering Gene in Cucumber,” Chinese Academy of Agricultural Sciences Dissertation, English abstract and p. 12: 4 pp. cited by applicant
      GenBank (2015) “Predicted: Cucumis sativus PHD finger protein Male Meiocyte Death 1 (LOC101209218), mRNA” NCBI Reference Sequence XM_011654407.2, Available online at https://www.ncbi.nlm.nih.gov/nuccore/1784863903: 3 pp. cited by applicant
    • Primary Examiner:
      Switzer, Juliet C
    • Attorney, Agent or Firm:
      Leydig, Voit & Mayer, Ltd.
    • Accession Number:
      edspgr.11319600