- Patent Number:
11833,116
- Appl. No:
17/411067
- Application Filed:
August 25, 2021
- Abstract:
The present invention discloses yeast powder rich in nicotinamide mononucleotide, a preparation method and uses thereof. The preparation method includes: preparing a first medium, a second medium, and a third medium; inoculating the first medium with yeast for fermentation to obtain a first fermentation broth; inoculating the second medium with the first fermentation broth for fermentation to obtain a second fermentation broth; inoculating the third medium with the second fermentation broth for fermentation to obtain a third fermentation broth; centrifuging the third fermentation broth to obtain a fermented product; and drying the fermented product to obtain the yeast powder. Components of the first medium, the second medium and the third medium include nicotinamide, tryptophan and niacin. The content of nicotinamide mononucleotide in the yeast powder is at least 5000 ppm.
- Inventors:
TCI CO., LTD. (Taipei, TW)
- Assignees:
TCI CO., LTD. (Taipei, TW)
- Claim:
1. A preparation method of yeast powder rich in nicotinamide mononucleotide, comprising: preparing a first medium, a second medium and a third medium, wherein components of the first medium, the second medium and the third medium comprise nicotinamide, tryptophan, and niacin; inoculating the first medium with yeast for fermentation to obtain a first fermentation broth; inoculating the second medium with the first fermentation broth for fermentation to obtain a second fermentation broth; inoculating the third medium with the second fermentation broth for fermentation to obtain a third fermentation broth; and centrifuging the third fermentation broth to obtain a fermented product, and drying the fermented product to obtain the yeast powder, wherein a content of the nicotinamide mononucleotide in the yeast powder is at least 5000 ppm.
- Claim:
2. The preparation method according to claim 1 , wherein the first medium is inoculated with the yeast at an inoculum amount of 5 vol %; the second medium is inoculated with the first fermentation broth at an inoculum amount of 6 vol %; and the third medium is inoculated with the second fermentation broth at an inoculum amount of 10 vol %.
- Claim:
3. The preparation method according to claim 1 , wherein a concentration of the nicotinamide is 0.01 wt % to 0.3 wt %, a concentration of the tryptophan is 0.1 wt % to 0.5 wt %, and a concentration of the niacin is 0.001 wt % to 0.06 wt %.
- Claim:
4. The preparation method according to claim 1 , wherein a volume ratio of the first medium to the second medium to the third medium is 3:50:500.
- Claim:
5. The preparation method according to claim 1 , wherein the yeast powder further comprises ubiquinone biosynthesis protein (COQ).
- Claim:
6. The preparation method according to claim 1 , wherein the components of the first medium, the second medium and the third medium further comprise a Solanum plant extract, an Aronia plant extract, a Musa plant extract, or any combination thereof.
- Patent References Cited:
3650898 March 1972 Nakayama et al.
20160287621 October 2016 Sinclair et al.
111093397 May 2020
111225657 June 2020
WO 95/01422 January 1995
2015069860 May 2015
2017022768 February 2017
2017200050 November 2017
2020097116 May 2020
- Other References:
Paalme et al., World J Microbiol Biotechnol, 2014; 30: 2351-2359 (Year: 2014). cited by examiner
The Production of Coenzyme Q10 in Microorganisms, Corinne P. Cluis et al., Reprogramming Microbial Metabolic Pathways, 2012, Chapter 15, p. 303-310. cited by applicant
- Assistant Examiner:
Jackson-Tongue, Lakia J
- Primary Examiner:
Gangle, Brian
- Attorney, Agent or Firm:
Wang, Chieh-Mei
- Accession Number:
edspgr.11833116
Processing Request
No Comments.