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DNA fragmentation index (DFI) as a measure of sperm quality and fertility in mice

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  • Additional Information
    • Publication Information:
      eScholarship, University of California, 2020.
    • Publication Date:
      2020
    • Abstract:
      Although thousands of genetically modified mouse strains have been cryopreserved by sperm freezing, the likelihood of cryorecovery success cannot be accurately predicted using conventional sperm parameters. The objective of the present study was to assess the extent to which measurement of a sperm DNA fragmentation index (DFI) can predict sperm quality and fertility after cryopreservation. Using a modified TUNEL assay, we measured and correlated the DFI of frozen-thawed sperm from 83 unique mutant mouse strains with sperm count, motility and morphology. We observed a linear inverse correlation between sperm DFI and sperm morphology and motility. Further, sperm DFI was significantly higher from males with low sperm counts compared to males with normal sperm counts (P
    • File Description:
      application/pdf
    • Rights:
      public
    • Accession Number:
      edssch.oai:escholarship.org:ark:/13030/qt2q88h9st