Profiling the immune response to Mycobacterium tuberculosis Beijing family infection: a perspective from the transcriptome.

Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101531386 Publication Model: Print Cited Medium: Internet ISSN: 2150-5608 (Electronic) Linking ISSN: 21505594 NLM ISO Abbreviation: Virulence Subsets: MEDLINE

Publication: 2015- : Philadelphia, PA : Taylor & Francis
Original Publication: Austin, Tex. : Landes Bioscience

Mycobacterium tuberculosis*/genetics ; Transcriptome* ; Tuberculosis*/immunology ; Tuberculosis*/microbiology; Animals ; Immunity ; Mice ; Mice, Inbred BALB C ; Necrosis

Tuberculosis continues to be an important public health problem. Particularly considering Beijing-family strains of Mycobacterium tuberculosis , which have been associated with drug-resistance and hypervirulence. The Beijing-like SIT190 (BL) is the most prevalent Beijing strain in Colombia. The pathogenic mechanism and immune response against this pathogen is unknown. Thus, we compared the course of pulmonary TB in BALB/c mice infected with Classical-Beijing strain 391 and BL strain 323. The disease course was different among infected animals with Classical-Beijing and BL strain. Mice infected with BL had a 100% mortality at 45 days post-infection (dpi), with high bacillary loads and massive pneumonia, whereas infected animals with Classical-Beijing survived until 60 dpi and showed extensive pneumonia and necrosis. Lung RNA extraction was carried out at early (day 3 dpi), intermediate (day 14 dpi), and late (days 28 and 60 dpi) time points of infection. Transcriptional analysis of infected mice with Classical-Beijing showed several over-expressed genes, associated with a pro-inflammatory profile, including those for coding for CCL3 and CCL4 chemokines, both biomarkers of disease severity. Conversely, mice infected with BL displayed a profile which included the over-expression of several genes associated with immune-suppression, including Nkiras, Dleu2, and Sphk2 , highlighting an anti-inflammatory milieu which would allow high bacterial replication followed by an intense inflammatory response. In summary, both Beijing strains induced a non-protective immune response which induced extensive tissue damage, BL strain induced rapidly extensive pneumonia and death, whereas Classical-Beijing strain produced slower extensive pneumonia later associated with extensive necrosis.

Comment in: Virulence. 2022 Dec;13(1):1-4. (PMID: 34753390)

Bioinformatics. 2015 Jan 15;31(2):166-9. (PMID: 25260700)
Bioinformatics. 2009 Jul 15;25(14):1754-60. (PMID: 19451168)
Nature. 2014 Jun 12;510(7504):288-92. (PMID: 24870241)
J Biol Chem. 1997 Dec 12;272(50):31221-4. (PMID: 9395445)
Pathog Glob Health. 2019 Dec;113(8):336-351. (PMID: 31903874)
Infect Genet Evol. 2010 Jul;10(5):678-81. (PMID: 20362076)
JAMA. 1998 Nov 18;280(19):1653. (PMID: 9831983)
Annu Rev Immunol. 2015;33:169-200. (PMID: 25493333)
Nucleic Acids Res. 2013 Jan;41(Database issue):D1228-33. (PMID: 23180781)
Blood. 2017 Nov 9;130(19):2092-2100. (PMID: 28807980)
J Biol Chem. 1998 May 15;273(20):12101-8. (PMID: 9575154)
J Biol Chem. 2005 Nov 4;280(44):37118-29. (PMID: 16118219)
Nat Immunol. 2002 Feb;3(2):151-8. (PMID: 11812992)
J Clin Microbiol. 2008 Aug;46(8):2692-9. (PMID: 18550737)
Nat Cell Biol. 2002 Jul;4(7):495-501. (PMID: 12055638)
PLoS Pathog. 2011 Mar;7(3):e1001307. (PMID: 21408618)
J Immunol. 2007 Nov 1;179(9):5644-8. (PMID: 17947634)
FEMS Immunol Med Microbiol. 1995 Sep;12(1):63-72. (PMID: 8580904)
Immunology. 2002 Jun;106(2):257-66. (PMID: 12047755)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
PLoS One. 2014 Apr 11;9(4):e94296. (PMID: 24728339)
Clin Exp Allergy. 2019 Nov;49(11):1418-1428. (PMID: 31264263)
J Clin Microbiol. 1997 Apr;35(4):907-14. (PMID: 9157152)
J Biol Chem. 2014 Jun 6;289(23):16085-99. (PMID: 24764305)
Nucleic Acids Res. 2017 Jul 3;45(W1):W130-W137. (PMID: 28472511)
PLoS One. 2008 Aug 06;3(8):e2880. (PMID: 18682727)
Cytokine Growth Factor Rev. 2003 Dec;14(6):467-77. (PMID: 14563349)
PLoS One. 2018 Aug 1;13(8):e0200632. (PMID: 30067763)
J Clin Microbiol. 1995 Dec;33(12):3234-8. (PMID: 8586708)
Emerg Infect Dis. 2002 Aug;8(8):843-9. (PMID: 12141971)
J Immunol. 1998 May 1;160(9):4190-8. (PMID: 9574519)
Front Endocrinol (Lausanne). 2019 Jun 06;10:351. (PMID: 31244771)
PLoS One. 2010 Aug 31;5(8):e12266. (PMID: 20824205)
Biol Cell. 2017 Feb;109(2):94-112. (PMID: 27673746)
N Engl J Med. 1998 Aug 20;339(8):520-32. (PMID: 9709046)
Nat Microbiol. 2018 Feb;3(2):181-188. (PMID: 29335553)
Int J Tuberc Lung Dis. 2000 Jul;4(7):673-83. (PMID: 10907771)
PLoS One. 2011;6(9):e23870. (PMID: 21931620)
Lancet Infect Dis. 2010 Feb;10(2):103-11. (PMID: 20113979)
Tuberculosis (Edinb). 2018 Jan;108:118-123. (PMID: 29523311)
Science. 2000 Feb 4;287(5454):869-73. (PMID: 10657303)
J Clin Microbiol. 2006 Dec;44(12):4498-510. (PMID: 17005759)
Infect Genet Evol. 2017 Oct;54:314-323. (PMID: 28734764)
Immunology. 1996 Sep;89(1):26-33. (PMID: 8911136)
Microbiol Spectr. 2017 Jun;5(3):. (PMID: 28597814)
Apoptosis. 2008 Apr;13(4):562-72. (PMID: 18330707)
Int J Mol Sci. 2020 Mar 17;21(6):. (PMID: 32192225)
Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Sep;1864(9):1235-1246. (PMID: 31128248)
Cancer Cell. 2010 Jan 19;17(1):28-40. (PMID: 20060366)
Sci Rep. 2015 Sep 28;5:14488. (PMID: 26412089)
Nat Immunol. 2008 Aug;9(8):880-6. (PMID: 18587400)
Genome Announc. 2015 Apr 30;3(2):. (PMID: 25931600)
Clin Exp Immunol. 2003 Jul;133(1):30-7. (PMID: 12823275)
Annu Rev Immunol. 2014;32:547-77. (PMID: 24655298)
J Biol Chem. 2012 Sep 7;287(37):30897-905. (PMID: 22952240)
J Clin Microbiol. 2009 Feb;47(2):335-44. (PMID: 19036933)
Cell Death Dis. 2018 May 24;9(6):624. (PMID: 29795378)
J Virol. 2015 Mar;89(5):2462-8. (PMID: 25428874)
Biomedica. 2004 Jun;24 Supp 1:73-9. (PMID: 15495574)
Nucleic Acids Res. 2019 Jul 2;47(W1):W234-W241. (PMID: 30931480)
Infect Immun. 2013 Oct;81(10):3750-6. (PMID: 23897611)
J Cell Mol Med. 2018 Sep;22(9):4076-4084. (PMID: 29961269)
Mol Cell Biol. 2004 Apr;24(7):3048-56. (PMID: 15024091)
J Immunol. 2004 Sep 15;173(6):3653-9. (PMID: 15356110)
Nucleic Acids Res. 2010 Jul;38(Web Server issue):W326-31. (PMID: 20457747)
Biochem Biophys Res Commun. 2010 Apr 2;394(2):387-92. (PMID: 20214888)
Emerg Infect Dis. 2006 May;12(5):736-43. (PMID: 16704829)

Keywords: Beijing; Beijing-like; Colombia; M. tuberculosis; immune response; transcriptomics; virulence

Date Created: 20210706 Date Completed: 20220125 Latest Revision: 20240402

20240402

PMC8265813

10.1080/21505594.2021.1936432

34228582