Novel Risk Associations between microRNA Polymorphisms and Gastric Cancer in a Chilean Population.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Stomach Neoplasms*/epidemiology ; Stomach Neoplasms*/etiology ; Stomach Neoplasms*/genetics; Biomarkers, Tumor/*genetics ; Helicobacter Infections/*complications ; MicroRNAs/*genetics; Adult ; Case-Control Studies ; Chile/epidemiology ; Female ; Genetic Predisposition to Disease ; Humans ; Male ; Middle Aged ; Polymorphism, Single Nucleotide

Gastric cancer (GC) is the fifth leading cause of cancer deaths in the world, with variations across geographical regions and ethnicities. Emerging evidence indicates that miRNA expression is dysregulated in GC and its polymorphisms may contribute to these variations, which has yet to be explored in Latin American populations. In a case-control study of 310 GC patients and 311 healthy donors from Chile, we assessed the association of 279 polymorphisms in 242 miRNA genes. Two novel polymorphisms were found to be associated with GC: rs4822739:C>G (miR-548j) and rs701213:T>C (miR-4427). Additionally, rs1553867776:T>TCCCCA (miR-4274) and rs12416605:C>T (miR-938) were associated with intestinal-type GC, and rs4822739:C>G (miR-548j) and rs1439619:T>G (miR-3175) with TNM I-II stage. The polymorphisms rs6149511:T> TGAAGGGCTCCA (miR-6891), rs404337:G>A (miR-8084), and rs1439619:T>G (miR-3175) were identified among H.pylori -infected GC patients and rs7500280:T>C (miR-4719) and rs1439619:T>G (miR-3175) were found among H. pylori cag PAI+ infected GC cases. Prediction analysis suggests that seven polymorphisms could alter the secondary structure of the miRNA, and the other one is located in the seed region of miR-938. Targets of miRNAs are enriched in GC pathways, suggesting a possible biological effect. In this study, we identified seven novel associations and replicated one previously described in Caucasian population. These findings contribute to the understanding of miRNA genetic polymorphisms in the GC pathogenesis.

Chin Med J (Engl). 2014;127(3):554-9. (PMID: 24451966)
Microb Pathog. 2016 Apr;93:44-55. (PMID: 26796299)
OMICS. 2012 May;16(5):284-7. (PMID: 22455463)
Genome Res. 2017 Mar;27(3):374-384. (PMID: 28087842)
Mol Cancer. 2008 Feb 27;7:22. (PMID: 18302799)
Cell. 2018 Mar 22;173(1):20-51. (PMID: 29570994)
Biosci Rep. 2021 Jan 29;41(1):. (PMID: 33319237)
Mol Genet Genomic Med. 2019 Aug;7(8):e832. (PMID: 31273931)
Gene. 2020 Feb 5;726:144173. (PMID: 31669639)
Mod Pathol. 2021 Mar;34(3):672-683. (PMID: 32973329)
Sci Rep. 2017 Jan 09;7:39528. (PMID: 28067243)
Nucleic Acids Res. 2019 Jan 8;47(D1):D155-D162. (PMID: 30423142)
Mol Oncol. 2016 Jun;10(6):838-49. (PMID: 26949125)
Annu Rev Med. 2019 Jan 27;70:353-367. (PMID: 30355265)
Arch Med Res. 2019 Jan;50(1):21-30. (PMID: 31101240)
Oncotarget. 2017 Nov 20;8(69):114344-114359. (PMID: 29371991)
Gigascience. 2015 Feb 25;4:7. (PMID: 25722852)
PLoS One. 2015 Nov 13;10(11):e0142387. (PMID: 26565624)
Nat Protoc. 2010 Sep;5(9):1564-73. (PMID: 21085122)
J Transl Med. 2018 Feb 23;16(1):38. (PMID: 29471858)
Anticancer Res. 2020 Mar;40(3):1495-1502. (PMID: 32132049)
Nat Rev Gastroenterol Hepatol. 2014 Nov;11(11):664-74. (PMID: 25134511)
Microrna. 2019;8(2):94-100. (PMID: 30514199)
Transl Gastroenterol Hepatol. 2019 Jul 29;4:55. (PMID: 31463414)
Nucleic Acids Res. 2012 Dec;40(22):11673-83. (PMID: 23034802)
Gut. 2015 Nov;64(11):1721-31. (PMID: 25385008)
Gut. 2020 Apr;69(4):748-763. (PMID: 32034004)
J Cell Biochem. 2019 Mar;120(3):3306-3314. (PMID: 30203457)
Eur J Clin Invest. 2020 Apr;50(4):e13203. (PMID: 31984489)
Pathol Oncol Res. 2017 Oct;23(4):899-905. (PMID: 28795345)
J Med Microbiol. 2002 Apr;51(4):305-311. (PMID: 11926735)
Arch Med Res. 2018 May;49(4):270-277. (PMID: 30274913)
Kaohsiung J Med Sci. 2021 Jan;37(1):20-26. (PMID: 32833340)
Cancer Causes Control. 2013 Feb;24(2):217-31. (PMID: 23224270)
Biol Res. 2021 Apr 20;54(1):13. (PMID: 33879265)
Nat Protoc. 2006;1(2):581-5. (PMID: 17406285)
Congenit Anom (Kyoto). 2014 Feb;54(1):12-21. (PMID: 24330020)
Cancers (Basel). 2020 Sep 24;12(10):. (PMID: 32987716)
Nat Commun. 2019 Nov 28;10(1):5436. (PMID: 31780650)

15130011 Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias; 1191928 Fondo Nacional de Desarrollo Científico y Tecnológico; 1151015 Fondo Nacional de Desarrollo Científico y Tecnológico

Keywords: Helicobacter pylori; cag pathogenicity island; gastric cancer; miRNA; polymorphism

0 (Biomarkers, Tumor)
0 (MicroRNAs)

Date Created: 20220111 Date Completed: 20220211 Latest Revision: 20220211

20231215

PMC8745138

10.3390/ijms23010467

35008894