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Antioxidant and antidiabetic flavonoids from the leaves of Dypsis pembana (H.E.Moore) Beentje & J.Dransf., Arecaceae: in vitro and molecular docking studies.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101761232 Publication Model: Electronic Cited Medium: Internet ISSN: 2662-7671 (Electronic) Linking ISSN: 26627671 NLM ISO Abbreviation: BMC Complement Med Ther Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, [2020]-
    • Subject Terms:
      Arecaceae* ; Cytochrome-c Peroxidase*; Hypoglycemic Agents/pharmacology ; Hypoglycemic Agents/chemistry ; Antioxidants/chemistry ; Kaempferols ; Molecular Docking Simulation ; Glycoside Hydrolase Inhibitors/pharmacology ; Glycoside Hydrolase Inhibitors/chemistry ; Plant Extracts/chemistry ; Flavonoids/chemistry ; alpha-Glucosidases/chemistry
    • Abstract:
      Background: Oxidative stress and diabetes are medical conditions that have a growing prevalence worldwide, significantly impacting our bodies. Thus, it is essential to develop new natural antioxidant and antidiabetic agents. Dypsis pembana (H.E.Moore) Beentje & J.Dransf (DP) is an ornamental palm of the family Arecaceae. This study aimed to broaden the understanding of this plant's biological properties by evaluating its in vitro antioxidant and antidiabetic activities.
      Methods: The in vitro antioxidant activities of the crude extract, fractions, and selected isolates were evaluated by DPPH method. While the in vitro antidiabetic activities of these samples were evaluated by assessing the degree of inhibition of α-glucosidase. Additionally, molecular docking analysis was performed to investigate the interactions of tested compounds with two potential targets, the cytochrome c peroxidase and alpha glucosidase.
      Results: The crude extract displayed the highest antioxidant activity (IC 50 of 11.56 µg/ml), whereas among the fractions, the EtOAc fraction was the most potent (IC 50 of 14.20 µg/ml). Among tested compounds, isoquercetrin (10) demonstrated the highest potency, with an IC 50 value of 3.30 µg/ml, followed by rutin (8) (IC 50 of 3.61 µg/ml). Regarding antidiabetic activity, the EtOAc (IC 50 of 60.4 µg/ml) and CH 2 Cl 2 fractions (IC 50 of 214.9 µg/ml) showed activity, while the other fractions did not demonstrate significant antidiabetic effects. Among tested compounds, kaempferol-3-O-neohesperidoside (9) showed the highest antidiabetic activity, with an IC 50 value of 18.38 µg/ml, followed by kaempferol (4) (IC 50 of 37.19 µg/ml). These experimental findings were further supported by molecular docking analysis, which revealed that isoquercetrin and kaempferol-3-O-neohesperidoside exhibited strong enzyme-binding affinities to the studied enzyme targets. This analysis provided insights into the structure-activity relationships among the investigated flavonol-O-glycosides.
      Conclusion: The biological and computational findings revealed that isoquercetrin and kaempferol-3-O-neohesperidoside have potential as lead compounds for inhibiting cytochrome c peroxidase and alpha glucosidase enzymes, respectively.
      (© 2023. The Author(s).)
    • References:
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    • Contributed Indexing:
      Keywords: Alpha glucosidase enzymes; Antidiabetic; Antioxidant; Arecaceae; Chrysalidocarpus Pembanus; Cytochrome c peroxidase; Dypsis Pembana; Flavonoid-O-glycoside; Flavonoids; Isoquercetrin; Kaempferol-3-O-neohesperidoside; Rutin
    • Accession Number:
      0 (Hypoglycemic Agents)
      0 (Antioxidants)
      0 (Kaempferols)
      0 (Glycoside Hydrolase Inhibitors)
      0 (Plant Extracts)
      0 (Flavonoids)
      EC 3.2.1.20 (alpha-Glucosidases)
      EC 1.11.1.5 (Cytochrome-c Peroxidase)
    • Publication Date:
      Date Created: 20231205 Date Completed: 20231207 Latest Revision: 20231208
    • Publication Date:
      20240105
    • Accession Number:
      PMC10698917
    • Accession Number:
      10.1186/s12906-023-04287-z
    • Accession Number:
      38053195