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Characterization of Sodium Channel Peptides Obtained from the Venom of the Scorpion Centruroides bonito .

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel : MDPI
    • Subject Terms:
      Venoms* ; Perciformes* ; Animals, Poisonous*; Humans ; Cricetinae ; Animals ; Horses ; Mice ; Scorpions ; Cricetulus ; Escherichia coli ; Phylogeny ; Antivenins ; Amino Acids ; Immunoglobulin Fragments ; Peptides
    • Abstract:
      Five peptides were isolated from the venom of the Mexican scorpion Centruroides bonito by chromatographic procedures (molecular weight sieving, ion exchange columns, and HPLC) and were denoted Cbo1 to Cbo5. The first four peptides contain 66 amino acid residues and the last one contains 65 amino acids, stabilized by four disulfide bonds, with a molecular weight spanning from about 7.5 to 7.8 kDa. Four of them are toxic to mice, and their function on human Na + channels expressed in HEK and CHO cells was verified. One of them (Cbo5) did not show any physiological effects. The ones toxic to mice showed that they are modifiers of the gating mechanism of the channels and belong to the beta type scorpion toxin (β-ScTx), affecting mainly the Nav1.6 channels. A phylogenetic tree analysis of their sequences confirmed the high degree of amino acid similarities with other known bona fide β-ScTx. The envenomation caused by this venom in mice is treated by using commercially horse antivenom available in Mexico. The potential neutralization of the toxic components was evaluated by means of surface plasmon resonance using four antibody fragments (10FG2, HV, LR, and 11F) which have been developed by our group. These antitoxins are antibody fragments of single-chain antibody type, expressed in E. coli and capable of recognizing Cbo1 to Cbo4 toxins to various degrees.
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    • Grant Information:
      303045 Consejo Nacional de Humanidades, Ciencias y Tecnologías; IN200323 DGAPA UNAM
    • Contributed Indexing:
      Keywords: Cbo1–Cbo5; Centruroides bonito; antivenoms; scorpion toxin; single-chain antibodies; voltage-gated sodium channels
    • Accession Number:
      0 (Venoms)
      0 (Antivenins)
      0 (Amino Acids)
      0 (Immunoglobulin Fragments)
      0 (Peptides)
    • Subject Terms:
      Centruroides
    • Publication Date:
      Date Created: 20240327 Date Completed: 20240328 Latest Revision: 20240330
    • Publication Date:
      20240330
    • Accession Number:
      PMC10974992
    • Accession Number:
      10.3390/toxins16030125
    • Accession Number:
      38535792