Abstract: Objective. To determine the presence of Mycobacterium avium subsp. paratuberculosis (MAP) using serological, microbiological, and molecular methods, to genotype MAP isolates, and to explore factors associated to MAP-seropositive status in a buffalo herd of Antioquia (Colombia). Materials and methods. The study was carried out to test fecal and serum samples from 21 asymptomatic adult buffaloes using culture and ELISA tests, respectively. Suspicious culture isolates were confirmed by MAP-IS900-PCR (IS900-PCR). The positive DNA was retested using MAP-IS900-quantative PCR (IS900-qPCR), and then, sub-typed using mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) and multilocus short sequence repeat (MLSSR). Results. At sampling, the average age of animals was 4.7 years, 90.5% (19/21) were females and 84.2% (16/19) were pregnant. The average body condition score was 4.2 (in a 1-to-5 scale). The average milk yield was 3.5 L/day/animal. No MAP-seropositive animals were detected. One dairy buffalo cow out of the 21 buffaloes was positive by fecal culture at week six of incubation and then confirmed by IS900-PCR. Sub-typing analysis revealed that the MAP isolate corresponded to the INMV 3 genotype by MIRU-VNTR. Conclusions. To the best of the author’s knowledge, this is the first report of MAP diagnosis and isolation in Colombian buffaloes and the first time an INMV 3 MAP-profile is found in this species the country. ; Objetivo. Determinar la presencia de Mycobacterium avium subsp. paratuberculosis (MAP) mediante métodos serológicos, microbiológicos y moleculares, para genotipar los aislamientos de MAP y explorar factores asociados a la seropositividad a MAP en un hato de búfalos del departamento de Antioquia (Colombia). Materiales y métodos. Se realizó un estudio transversal para analizar muestras fecales y de suero de 21 búfalas adultas asintomáticas mediante cultivo y ELISA, respectivamente. Los cultivos aislados sospechosos se confirmaron mediante PCR de MAP-IS900 (IS900-PCR). ...
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