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Mitochondrial genome of the fluke pond snail, Austropeplea cf. brazieri (Gastropoda: Lymnaeidae).

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central
    • Subject Terms:
      Genome, Mitochondrial*/genetics ; Phylogeny* ; Snails*/parasitology; Animals ; Australia ; Fasciola hepatica/genetics ; Fasciola hepatica/classification ; Electron Transport Complex IV/genetics ; Disease Vectors ; Sequence Analysis, DNA
    • Abstract:
      Background: Lymnaeid snails of the genus Austropeplea are an important vector of the liver fluke (Fasciola hepatica), contributing to livestock production losses in Australia and New Zealand. However, the species status within Austropeplea is ambiguous due to heavy reliance on morphological analysis and a relative lack of genetic data. This study aimed to characterise the mitochondrial genome of A. cf. brazieri, an intermediate host of liver fluke in eastern Victoria.
      Methods: The mitochondrial genome was assembled and annotated from a combination of second- and third-generation sequencing data. For comparative purposes, we performed phylogenetic analyses of the concatenated nucleotide sequences of the mitochondrial protein-coding genes, cytochrome c oxidase subunit 1 and 16S genes.
      Results: The assembled mt genome was 13,757 base pairs and comprised 37 genes, including 13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. The mt genome length, gene order and nucleotide compositions were similar to related species of lymnaeids. Phylogenetic analyses of the mt nucleotide sequences placed A. cf. brazieri within the same clade as Orientogalba ollula with strong statistical supports. Phylogenies of the cox1 and 16S mt sequences were constructed due to the wide availability of these sequences representing the lymnaeid taxa. As expected in both these phylogenies, A. cf. brazieri clustered with other Austropeplea sequences, but the nodal supports were low.
      Conclusions: The representative mt genome of A. cf. brazieri should provide a useful resource for future molecular, epidemiology and parasitological studies of this socio-economically important lymnaeid species.
      (© 2024. The Author(s).)
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    • Grant Information:
      DP230100270 Australian Research Council; DP230100270 Australian Research Council; DP230100270 Australian Research Council; DP230100270 Australian Research Council; DP230100270 Australian Research Council; DP230100270 Australian Research Council; DP230100270 Australian Research Council; DP230100270 Australian Research Council
    • Contributed Indexing:
      Keywords: Austropeplea; Fasciola hepatica; Australia; Intermediate host; Mitochondrial genome; Snail
    • Accession Number:
      EC 1.9.3.1 (Electron Transport Complex IV)
    • Publication Date:
      Date Created: 20240703 Date Completed: 20240703 Latest Revision: 20240705
    • Publication Date:
      20240705
    • Accession Number:
      PMC11218368
    • Accession Number:
      10.1186/s13071-024-06358-7
    • Accession Number:
      38956636